Raster image cross-correlation analysis for spatiotemporal visualization of intracellular degradation activities against exogenous DNAs

Raster image cross-correlation analysis for spatiotemporal visualization of intracellular degradation activities against exogenous DNAs

Reducing intracellular DNA degradation is critical to enhance the efficiency of gene therapy. Exogenous DNA incorporation into cells is strictly blocked by the defense machinery of intracellular nuclease activity. Raster image correlation spectroscopy (RICS) and raster image cross-correlation spectroscopy (cross-correlation RICS; ccRICS) are image-based correlation methods. These powerful tools...

Raster Image Correlation Spectroscopic Analysis of Intranuclear Molecular Dynamics

In contrast to conventional epi-illumination microscopy in confocal laser scanning microscopy images are acquired sequentially either in point scanning or line scanning mode. This scanning approach therefore contains spatio-temporal information, which can be used to obtain insight into molecular dynamics, e.g. diffusion coefficients of fluorescently labeled probes. Raster Image Correlation Spec...

Raster image correlation spectroscopy and number and brightness analysis.

The raster image correlation spectroscopy (RICS) and number and molecular brightness (N&B) methods are used to measure molecular diffusion in complex biological environments such as the cell interior, detect the formation of molecular aggregates, establish the stoichiometry of the aggregates, spatially map the number of mobile molecules, and quantify the relative fraction of molecules participa...

New Insight from Using Spatiotemporal Image Correlation in Prenatal Screening of Fetal Conotruncal Defects

Spatiotemporal Analysis and Visualization for Environmental Studies

An abundance of information on earth resources is being collected daily such as remotely sensed imagery, Global Positioning System (GPS) surveys, aerial photographs and ground truth data. Interpreted by resource experts and compiled into geographic information system (GIS) digital databases, these data are increasingly available for viewing and distribution over the world wide web. With such a ...